Role of RUNX1 (AML1) Dysregulation in Juvenile Myelomonocytic LeukemiaDescription of Major Goals
This proposal aims to further elucidate the molecular pathogenesis of an aggressive cancer of young children, with the goal of identifying improved and less-toxic treatments for this disorder
Runx1 Regulatory Mechanisms in Hematopoietic Stem Cell Ontogeny and MaintenanceDescription of Major Goals
The main goal of this study is to further elucidate the regulatory mechanisms that govern RUNX1’s activity in hematopoietic stem cell ontogeny and maintenance. It examines interactions between RUNX1 and Bmi1, and the role of src-family kinase mediated RUNX1 tyrosine phosphorylation.
Inhibition of Runx1 Tyrosine Phosphorylation in the Treatment of Human LeukemiaDescription of Major Goals
The major goal of this study is to test whether clinically available src family tyrosine kinase inhibitors can block Runx1 tyrosine phosphorylation and enhance its activity.
Role ASXL1 Mutations in Human Myelodysplastic Syndrome and LeukemiaDescription of Major Goals
The aims of this study are to identify novel ASXL1 interacting proteins to further understand its function in hematopoiesis, and to test the hypothesis that mutant ASXL1 proteins associated with human leukemia have dominant negative activity.
Proteomic Approach to Further Understanding the Role of Runx-1 in MyelodysplasiaDescription of Major Goals
The goals of this study were to purify and characterize Runx-1 containing multiprotein complexes in hematopoietic cells and examine the effects of myelodysplastic syndrome (MDS) associated Runx-1 mutations on observed protein-protein interactions.
Proteomic Study of Megakaryocyte Transcriptional ControlDescription of Major Goals
This goal of this study is to isolate GATA-1 containing multiprotein complexes from megakaryocytes, identify the components, and assess their functional significance in megakaryopoiesis.
Transcriptional Regulation of MegakaryopoiesisDescription of Major Goals
The main goal of this study was to further understand the transcriptional regulation of megakaryopoiesis by identifying novel GATA-1 associated proteins and assessing their functional significance in megakaryopoiesis.
Role of FOG-1 in HematopoiesisDescription of Major Goals
The major goal of this project is to define the mechanism of action of the transcriptional cofactor FOG-1 in hematopoiesis through a structure/function analysis.